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BMCBI
2011

AMDORAP: Non-targeted metabolic profiling based on high-resolution LC-MS

10 years 3 months ago
AMDORAP: Non-targeted metabolic profiling based on high-resolution LC-MS
Background: Liquid chromatography-mass spectrometry (LC-MS) utilizing the high-resolution power of an orbitrap is an important analytical technique for both metabolomics and proteomics. Most important feature of the orbitrap is excellent mass accuracy. Thus, it is necessary to convert raw data to accurate and reliable m/z values for metabolic fingerprinting by high-resolution LC-MS. Results: In the present study, we developed a novel, easy-to-use and straightforward m/z detection method, AMDORAP. For assessing the performance, we used real biological samples, Bacillus subtilis strains 168 and MGB874, in the positive mode by LC-orbitrap. For 14 identified compounds by measuring the authentic compounds, we compared obtained m/z values with other LC-MS processing tools. The errors by AMDORAP were distributed within ±3 ppm and showed the best performance in m/z value accuracy. Conclusions: Our method can detect m/z values of biological samples much more accurately than other LC-MS analys...
Hiroki Takahashi, Takuya Morimoto, Naotake Ogasawa
Added 24 Aug 2011
Updated 24 Aug 2011
Type Journal
Year 2011
Where BMCBI
Authors Hiroki Takahashi, Takuya Morimoto, Naotake Ogasawara, Shigehiko Kanaya
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