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BMCBI
2007

Assessing the ability of sequence-based methods to provide functional insight within membrane integral proteins: a case study an

13 years 3 months ago
Assessing the ability of sequence-based methods to provide functional insight within membrane integral proteins: a case study an
Background: Efforts to predict functional sites from globular proteins is increasingly common; however, the most successful of these methods generally require structural insight. Unfortunately, despite several recent technological advances, structural coverage of membrane integral proteins continues to be sparse. ConSequently, sequence-based methods represent an important alternative to illuminate functional roles. In this report, we critically examine the ability of several computational methods to provide functional insight within two specific areas. First, can phylogenomic methods accurately describe the functional diversity across a membrane integral protein family? And second, can sequence-based strategies accurately predict key functional sites? Due to the presence of a recently solved structure and a vast amount of experimental mutagenesis data, the neurotransmitter/Na+ symporter (NSS) family is an ideal model system to assess the quality of our predictions. Results: The raw NS...
Dennis R. Livesay, Patrick D. Kidd, Sepehr Eskanda
Added 12 Dec 2010
Updated 12 Dec 2010
Type Journal
Year 2007
Where BMCBI
Authors Dennis R. Livesay, Patrick D. Kidd, Sepehr Eskandari, Usman Roshan
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