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TASE
2010
IEEE

Automated Selection and Placement of Single Cells Using Vision-Based Feedback Control

9 years 6 months ago
Automated Selection and Placement of Single Cells Using Vision-Based Feedback Control
Abstract--We present a robotic manipulation system for automated selection and transfer of individual living cells to analysis locations. We begin with a commonly used cell transfer technique using glass capillary micropipettes to aspirate and release living cells suspended in liquid growth media. Using vision-based feedback and closed-loop process control, two individual three-axis robotic stages position the micropipette tip in proximity to the cell of interest. The cell is aspirated and the tip is moved to a target location where the cell is dispensed. Computer vision is used to monitor and inspect the success of the dispensing process. In our initial application, the target cell destination is a microwell etched in a fused silica substrate. The system offers a robust and flexible technology for cell selection and manipulation. Applications for this technology include embryonic stem cells transfer, blastomere biopsy, cell patterning, and cell surgery. Note to Practitioners--The need...
Y. H. Anis, Mark Holl, Deirdre R. Meldrum
Added 21 May 2011
Updated 21 May 2011
Type Journal
Year 2010
Where TASE
Authors Y. H. Anis, Mark Holl, Deirdre R. Meldrum
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