Sciweavers

Share
ISBI
2008
IEEE

Blind deconvolution for diffraction-limited fluorescence microscopy

12 years 9 months ago
Blind deconvolution for diffraction-limited fluorescence microscopy
Optical Sections of biological samples obtained from a fluorescence Confocal Laser Scanning Microscopes (CLSM) are often degraded by out-of-focus blur and photon counting noise. Such physical constraints on the observation are a result of the diffraction-limited nature of the optical system, and the reduced amount of light detected by the photomultiplier respectively. Hence, the image stacks can benefit from postprocessing restoration methods based on deconvolution. The parameters of the acquisition system's Point Spread Function (PSF) may vary during the course of experimentation, and so they have to be estimated directly from the observation data. We describe here an alternate minimization algorithm for the simultaneous blind estimation of the specimen 3D distribution of fluorescent sources and the PSF. Experimental results on real data show that the algorithm provides very good deconvolution results in comparison to theoretical microscope PSF models.
Praveen Pankajakshan, Bo Zhang, Laure Blanc-F&eacu
Added 20 Nov 2009
Updated 20 Nov 2009
Type Conference
Year 2008
Where ISBI
Authors Praveen Pankajakshan, Bo Zhang, Laure Blanc-Féraud, Zvi Kam, Jean-Christophe Olivo-Marin, Josiane Zerubia
Comments (0)
books