Sciweavers

Share
BMCBI
2010

A boundary delimitation algorithm to approximate cell soma volumes of bipolar cells from topographical data obtained by scanning

10 years 1 months ago
A boundary delimitation algorithm to approximate cell soma volumes of bipolar cells from topographical data obtained by scanning
Background: Cell volume determination plays a pivotal role in the investigation of the biophysical mechanisms underlying various cellular processes. Whereas light microscopy in principle enables one to obtain three dimensional data, the reconstruction of cell volume from z-stacks is a time consuming procedure. Thus, three dimensional topographic representations of cells are easier to obtain by scanning probe microscopical measurements. Results: We present a method of separating the cell soma volume of bipolar cells in adherent cell cultures from the contributions of the cell processes from data obtained by scanning ion conductance microscopy. Soma volume changes between successive scans obtained from the same cell can then be computed even if the cell is changing its position within the observed area. We demonstrate that the estimation of the cell volume on the basis of the width and the length of a cell may lead to erroneous determination of cell volume changes. Conclusions: We provi...
Patrick Happel, Kerstin Moller, Ralf Kunz, Irmgard
Added 08 Dec 2010
Updated 08 Dec 2010
Type Journal
Year 2010
Where BMCBI
Authors Patrick Happel, Kerstin Moller, Ralf Kunz, Irmgard D. Dietzel
Comments (0)
books