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BMCBI
2005

Construction and validation of the APOCHIP, a spotted oligo-microarray for the study of beta-cell apoptosis

11 years 9 months ago
Construction and validation of the APOCHIP, a spotted oligo-microarray for the study of beta-cell apoptosis
Background: Type 1 diabetes mellitus (T1DM) is a autoimmune disease caused by a long-term negative balance between immune-mediated beta-cell damage and beta-cell repair/regeneration. Following immune-mediated damage the beta-cell fate depends on several genes up- or downregulated in parallel and/or sequentially. Based on the information obtained by the analysis of several microarray experiments of beta-cells exposed to pro-apoptotic conditions (e.g. double stranded RNA (dsRNA) and cytokines), we have developed a spotted rat oligonucleotide microarray, the APOCHIP, containing 60-mer probes for 574 genes selected for the study of betacell apoptosis. Results: The APOCHIP was validated by a combination of approaches. First we performed an internal validation of the spotted probes based on a weighted linear regression model using dilution series experiments. Second we profiled expression measurements in ten dissimilar rat RNA samples for 515 genes that were represented on both the spotted ...
Nils E. Magnusson, Alessandra K. Cardozo, Mogens K
Added 15 Dec 2010
Updated 15 Dec 2010
Type Journal
Year 2005
Where BMCBI
Authors Nils E. Magnusson, Alessandra K. Cardozo, Mogens Kruhøffer, Decio L. Eizirik, Torben F. Ørntoft, Jens L. Jensen
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