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Extended Depth-of-Focus for Multi-Channel Microscopy Images: A Complex Wavelet Approach

12 years 6 months ago
Extended Depth-of-Focus for Multi-Channel Microscopy Images: A Complex Wavelet Approach
Microscopy imaging often suffers from limited depth-offocus. However, the specimen can be `optically sectioned' by moving the object along the optical axis; different areas appear in focus in different images. Extended depthof-focus is a fusion algorithm that combines those images into one single sharp composite. One promising method is based on the wavelet transform. In this paper, we show how the wavelet-based image fusion technique can be improved and easily extended to multi-channel data. First, we propose the use of complex-valued wavelet bases, which seem to outperform traditional real-valued wavelet transforms. Second, we introduce a way to apply this technique for multi-channel images that suppresses artifacts and does not introduce false colors, an important requirement for multi-channel fluorescence microscopy imaging. We evaluate our method on simulated image stacks and give results relevant to biological imaging.
Brigitte Forster, Dimitri Van De Ville, Jesse Bere
Added 20 Nov 2009
Updated 20 Nov 2009
Type Conference
Year 2004
Where ISBI
Authors Brigitte Forster, Dimitri Van De Ville, Jesse Berent, Daniel Sage, Michael Unser
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