An Integrated Circuit for Chip-Based Analysis of Enzyme Kinetics and Metabolite Quantification

—We have created a novel chip-based diagnostic tools based upon quantification of metabolites using enzymes specific for their chemical conversion. Using this device we show for the first time that a solid-state circuit can be used to measure enzyme kinetics and calculate the Michaelis-Menten constant. Substrate concentration dependency of enzyme reaction rates is central to this aim. Ion-sensitive field effect transistors (ISFET) are excellent transducers for biosensing applications that are reliant upon enzyme assays, especially since they can be fabricated using mainstream microelectronics technology to ensure low unit cost, mass-manufacture, scaling to make many sensors and straightforward miniaturisation for use in point-of-care devices. Here, we describe an integrated ISFET array comprising 216 sensors. The device was fabricated with a complementary metal oxide semiconductor (CMOS) process. Unlike traditional CMOS ISFET sensors that use the Si3N4 passivation of the foundry for ...

• Type: Journal
• Year: 2016
• Where: TBCAS
• Authors: Boon Chong Cheah, Alasdair Iain Macdonald, Christopher Martin, Angelos J. Streklas, Gordon Campbell, Mohammed Al-Rawhani, Balázs Németh, James P. Grant, Michael P. Barrett, David R. S. Cumming
• Keywords: TBCAS 2016