Sciweavers

BMCBI
2008

Integrated multi-level quality control for proteomic profiling studies using mass spectrometry

13 years 3 months ago
Integrated multi-level quality control for proteomic profiling studies using mass spectrometry
Background: Proteomic profiling using mass spectrometry (MS) is one of the most promising methods for the analysis of complex biological samples such as urine, serum and tissue for biomarker discovery. Such experiments are often conducted using MALDI-TOF (matrix-assisted laser desorption/ionisation time-of-flight) and SELDI-TOF (surface-enhanced laser desorption/ ionisation time-of-flight) MS. Using such profiling methods it is possible to identify changes in protein expression that differentiate disease states and individual proteins or patterns that may be useful as potential biomarkers. However, the incorporation of quality control (QC) processes that allow the identification of low quality spectra reliably and hence allow the removal of such data before further analysis is often overlooked. In this paper we describe rigorous methods for the assessment of quality of spectral data. These procedures are presented in a user-friendly, webbased program. The data obtained post-QC is then...
David A. Cairns, David N. Perkins, Anthea J. Stanl
Added 09 Dec 2010
Updated 09 Dec 2010
Type Journal
Year 2008
Where BMCBI
Authors David A. Cairns, David N. Perkins, Anthea J. Stanley, Douglas Thompson, Jennifer H. Barrett, Peter J. Selby, Rosamonde E. Banks
Comments (0)