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CEC
2009
IEEE

Intensity isotherms and distributions on oligonucleotide microarrays

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Intensity isotherms and distributions on oligonucleotide microarrays
We describe a physico-chemical model relating measured fluorescence intensities on oligonucleotide microarrays to the underlying specific target concentration in the hybridized solution via a hyperbolic isotherm response function. The distribution of fluorescence intensities for a complete microarray is analysed in the light of this model. Results indicate that the majority of signals in a typical microarray experiment, though not those of the most highly expressed genes, belong to the low concentration, linear part of the isotherm. Nevertheless, recognising the existence of the asymptotic saturation part of the isotherm is important for interpreting this distribution over the entire intensity range.
Conrad J. Burden
Added 20 May 2010
Updated 20 May 2010
Type Conference
Year 2009
Where CEC
Authors Conrad J. Burden
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