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BMCBI
2005

MASQOT: a method for cDNA microarray spot quality control

8 years 8 months ago
MASQOT: a method for cDNA microarray spot quality control
Background: cDNA microarray technology has emerged as a major player in the parallel detection of biomolecules, but still suffers from fundamental technical problems. Identifying and removing unreliable data is crucial to prevent the risk of receiving illusive analysis results. Visual assessment of spot quality is still a common procedure, despite the time-consuming work of manually inspecting spots in the range of hundreds of thousands or more. Results: A novel methodology for cDNA microarray spot quality control is outlined. Multivariate discriminant analysis was used to assess spot quality based on existing and novel descriptors. The presented methodology displays high reproducibility and was found superior in identifying unreliable data compared to other evaluated methodologies. Conclusion: The proposed methodology for cDNA microarray spot quality control generates non-discrete values of spot quality which can be utilized as weights in subsequent analysis procedures as well as to ...
Max Bylesjö, Daniel Eriksson, Andreas Sjö
Added 15 Dec 2010
Updated 15 Dec 2010
Type Journal
Year 2005
Where BMCBI
Authors Max Bylesjö, Daniel Eriksson, Andreas Sjödin, Michael Sjöström, Stefan Jansson, Henrik Antti, Johan Trygg
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