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BMCBI
2011

MRCQuant- an accurate LC/MS relative isotopic quantification algorithm on TOF instruments

9 years 11 months ago
MRCQuant- an accurate LC/MS relative isotopic quantification algorithm on TOF instruments
Background: Relative isotope abundance quantification, which can be used for peptide identification and differential peptide quantification, plays an important role in liquid chromatography-mass spectrometry (LC-MS)based proteomics. However, several major issues exist in the relative isotopic quantification of peptides on time-offlight (TOF) instruments: LC peak boundary detection, thermal noise suppression, interference removal and mass drift correction. We propose to use the Maximum Ratio Combining (MRC) method to extract MS signal templates for interference detection/removal and LC peak boundary detection. In our method, MRCQuant, MS templates are extracted directly from experimental values, and the mass drift in each LC-MS run is automatically captured and compensated. We compared the quantification accuracy of MRCQuant to that of another representative LC-MS quantification algorithm (msInspect) using datasets downloaded from a public data repository. Results: MRCQuant showed sign...
William H. Haskins, Konstantinos Petritis, Jianqiu
Added 12 May 2011
Updated 12 May 2011
Type Journal
Year 2011
Where BMCBI
Authors William H. Haskins, Konstantinos Petritis, Jianqiu Zhang
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