Nanopore current transduction analysis of protein binding to non-terminal and terminal DNA regions: analysis of transcription fa

Background: Synthetic transcription factors (STFs) promise to offer a powerful new therapeutic against Cancer, AIDS, and genetic disease. Currently, 10% of drugs are of this type, including salicylate and tamoxifen. STFs that can appropriately target (and release) their transcription factor binding sites on native genomic DNA provide a means to directly influence cellular mRNA production. An effective mechanism for screening amongst transcription factor (TF) candidates would itself be highly valued, and such may be possible with nanopore cheminformatics methods. Results: It is hypothesized that binding targets on channel-captured molecules, that are well away from the channel-captured region, can be monitored insofar as their binding status, or history, is concerned. The first set of experiments we perform to explore this "transduction" hypothesis involve non-terminal dsDNA binding to protein (DNA TATA box receptor binding to TBP), where we show new experimental results and ...

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Stephen Winters-Hilt, Amanda Davis, Iftekhar Amin, Eric Morales
• Keywords: BMCBI 2007, Native Genomic Dna, Synthetic Transcription Factors, Transcription Factor