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BMCBI
2004

prot4EST: Translating Expressed Sequence Tags from neglected genomes

8 years 11 months ago
prot4EST: Translating Expressed Sequence Tags from neglected genomes
Background: The genomes of an increasing number of species are being investigated through generation of expressed sequence tags (ESTs). However, ESTs are prone to sequencing errors and typically define incomplete transcripts, making downstream annotation difficult. Annotation would be greatly improved with robust polypeptide translations. Many current solutions for EST translation require a large number of full-length gene sequences for training purposes, a resource that is not available for the majority of EST projects. Results: As part of our ongoing EST programs investigating these "neglected" genomes, we have developed a polypeptide prediction pipeline, prot4EST. It incorporates freely available software to produce final translations that are more accurate than those derived from any single method. We show that this integrated approach goes a long way to overcoming the deficit in training data. Conclusions: prot4EST provides a portable EST translation solution and can be...
James Wasmuth, Mark L. Blaxter
Added 16 Dec 2010
Updated 16 Dec 2010
Type Journal
Year 2004
Where BMCBI
Authors James Wasmuth, Mark L. Blaxter
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