Robust Inter-slice Intensity Normalization Using Histogram Scale-Space Analysis

Abstract. This paper presents a robust method to correct for intensity differences across a series of aligned stained histological slices. The method is made up of two steps. First, for each slice, a scale-space analysis of the histogram provides a set of alternative interpretations in terms of tissue classes. Each of these interpretations can lead to a different classification of the related slice. A simple heuristics selects for each slice the most plausible interpretation. Then, an iterative procedure refines the interpretation selections across the series in order to maximize a score measuring the spatial consistency of the classifications across contiguous slices. Results are presented for a series of 121 baboon slices.

• Type: Conference
• Year: 2004
• Where: MICCAI
• Authors: Julien Dauguet, Jean-Francois Mangin, Thierry Delzescaux, Vincent Frouin
• Keywords: Baboon Slices, Contiguous Slices, Histological Slices, Medical Imaging, MICCAI 2004