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BMCBI
2007

In silico identification of NF-kappaB-regulated genes in pancreatic beta-cells

9 years 5 months ago
In silico identification of NF-kappaB-regulated genes in pancreatic beta-cells
Background: Pancreatic beta-cells are the target of an autoimmune attack in type 1 diabetes mellitus (T1DM). This is mediated in part by cytokines, such as interleukin (IL)-1β and interferon (IFN)-γ. These cytokines modify the expression of hundreds of genes, leading to beta-cell dysfunction and death by apoptosis. Several of these cytokine-induced genes are potentially regulated by the IL-1β-activated transcription factor (TF) nuclear factor (NF)-κB, and previous studies by our group have shown that cytokine-induced NF-κB activation is pro-apoptotic in betacells. To identify NF-κB-regulated gene networks in beta-cells we presently used a discriminant analysis-based approach to predict NF-κB responding genes on the basis of putative regulatory elements. Results: The performance of linear and quadratic discriminant analysis (LDA, QDA) in identifying NF-κB-responding genes was examined on a dataset of 240 positive and negative examples of NFκB regulation, using stratified cross...
Najib Naamane, Jacques van Helden, Decio L. Eiziri
Added 08 Dec 2010
Updated 08 Dec 2010
Type Journal
Year 2007
Where BMCBI
Authors Najib Naamane, Jacques van Helden, Decio L. Eizirik
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