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BMCBI
2007

In situ analysis of cross-hybridisation on microarrays and the inference of expression correlation

11 years 9 months ago
In situ analysis of cross-hybridisation on microarrays and the inference of expression correlation
Background: Microarray co-expression signatures are an important tool for studying gene function and relations between genes. In addition to genuine biological co-expression, correlated signals can result from technical deficiencies like hybridization of reporters with off-target transcripts. An approach that is able to distinguish these factors permits the detection of more biologically relevant co-expression signatures. Results: We demonstrate a positive relation between off-target reporter alignment strength and expression correlation in data from oligonucleotide genechips. Furthermore, we describe a method that allows the identification, from their expression data, of individual probe sets affected by offtarget hybridization. Conclusion: The effects of off-target hybridization on expression correlation coefficients can be substantial, and can be alleviated by more accurate mapping between microarray reporters and the target transcriptome. We recommend attention to the mapping for ...
Tineke Casneuf, Yves Van de Peer, Wolfgang Huber
Added 08 Dec 2010
Updated 08 Dec 2010
Type Journal
Year 2007
Where BMCBI
Authors Tineke Casneuf, Yves Van de Peer, Wolfgang Huber
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