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BMCBI
2007

Statistical tools for transgene copy number estimation based on real-time PCR

10 years 1 months ago
Statistical tools for transgene copy number estimation based on real-time PCR
Background: As compared with traditional transgene copy number detection technologies such as Southern blot analysis, real-time PCR provides a fast, inexpensive and high-throughput alternative. However, the real-time PCR based transgene copy number estimation tends to be ambiguous and subjective stemming from the lack of proper statistical analysis and data quality control to render a reliable estimation of copy number with a prediction value. Despite the recent progresses in statistical analysis of real-time PCR, few publications have integrated these advancements in realtime PCR based transgene copy number determination. Results: Three experimental designs and four data quality control integrated statistical models are presented. For the first method, external calibration curves are established for the transgene based on serially-diluted templates. The Ct number from a control transgenic event and putative transgenic event are compared to derive the transgene copy number or zygosity...
Joshua S. Yuan, Jason N. Burris, Nathan R. Stewart
Added 12 Dec 2010
Updated 12 Dec 2010
Type Journal
Year 2007
Where BMCBI
Authors Joshua S. Yuan, Jason N. Burris, Nathan R. Stewart, Ayalew Mentewab, C. Neal Stewart Jr.
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