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BMCBI
2010

Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing

10 years 2 months ago
Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
Background: OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality. Results: We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions. Conclusions: A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research. Background Most proteomics workflows from complex biological matrices require extensive sample processing at peptide or protein level to increase identification coverage. Recently, due to its separation capabilities, ease of use and relatively low cost, OFFGEL isoelectric focussing (IEF) has become a popular tool to fractionate proteins and peptides...
David-Olivier D. Azulay, Hendrik Neubert, Mireia F
Added 08 Dec 2010
Updated 08 Dec 2010
Type Journal
Year 2010
Where BMCBI
Authors David-Olivier D. Azulay, Hendrik Neubert, Mireia Fernández Ocaña
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