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BMCBI
2008
105views more  BMCBI 2008»
13 years 3 months ago
A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards
Background: In real-time quantitative PCR studies using absolute plasmid DNA standards, a calibration curve is developed to estimate an unknown DNA concentration. However, potenti...
Mano Sivaganesan, Shawn Seifring, Manju Varma, Ric...
BMCBI
2010
143views more  BMCBI 2010»
13 years 3 months ago
Shape based kinetic outlier detection in real-time PCR
Background: Real-time PCR has recently become the technique of choice for absolute and relative nucleic acid quantification. The gold standard quantification method in real-time P...
Davide Sisti, Michele Guescini, Marco B. L. Rocchi...