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» Improvement in the Reproducibility and Accuracy of DNA Micro...
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BMCBI
2006
127views more  BMCBI 2006»
13 years 4 months ago
Improvement in the Reproducibility and Accuracy of DNA Microarray Quantification by Optimizing Hybridization Conditions
Background: DNA microarrays, which have been increasingly used to monitor mRNA transcripts at a global level, can provide detailed insight into cellular processes involved in resp...
Tao Han, Cathy D. Melvin, Leming M. Shi, William S...
BMCBI
2010
105views more  BMCBI 2010»
13 years 4 months ago
Effects of scanning sensitivity and multiple scan algorithms on microarray data quality
Background: Maximizing the utility of DNA microarray data requires optimization of data acquisition through selection of an appropriate scanner setting. To increase the amount of ...
Andrew Williams, Errol M. Thomson
BMCBI
2010
108views more  BMCBI 2010»
12 years 11 months ago
Preferred analysis methods for Affymetrix GeneChips. II. An expanded, balanced, wholly-defined spike-in dataset
Background: Concomitant with the rise in the popularity of DNA microarrays has been a surge of proposed methods for the analysis of microarray data. Fully controlled "spike-i...
Qianqian Zhu, Jeffrey C. Miecznikowski, Marc S. Ha...
BMCBI
2008
99views more  BMCBI 2008»
13 years 4 months ago
A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition
Background: Real-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnos...
Michele Guescini, Davide Sisti, Marco B. L. Rocchi...