Background: Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) method produces allelespecific DNA bands of different lengths by adding four designed primers an...
Background: Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a f...
Background: A new algorithm for assessing similarity between primer and template has been developed based on the hypothesis that annealing of primer to template is an information ...