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BMCBI
2007
174views more  BMCBI 2007»
13 years 4 months ago
Normalization method for metabolomics data using optimal selection of multiple internal standards
Background: Success of metabolomics as the phenotyping platform largely depends on its ability to detect various sources of biological variability. Removal of platform-specific so...
Marko Sysi-Aho, Mikko Katajamaa, Laxman Yetukuri, ...
BMCBI
2005
85views more  BMCBI 2005»
13 years 4 months ago
Processing methods for differential analysis of LC/MS profile data
Background: Liquid chromatography coupled to mass spectrometry (LC/MS) has been widely used in proteomics and metabolomics research. In this context, the technology has been incre...
Mikko Katajamaa, Matej Oresic
BMCBI
2005
137views more  BMCBI 2005»
13 years 4 months ago
A standard curve based method for relative real time PCR data processing
Background: Currently real time PCR is the most precise method by which to measure gene expression. The method generates a large amount of raw numerical data and processing may no...
Alexey Larionov, Andreas Krause, William Miller
BMCBI
2010
106views more  BMCBI 2010»
13 years 4 months ago
Selection of optimal reference genes for normalization in quantitative RT-PCR
Background: Normalization in real-time qRT-PCR is necessary to compensate for experimental variation. A popular normalization strategy employs reference gene(s), which may introdu...
Inna Chervoneva, Yanyan Li, Stephanie Schulz, Sean...
BMCBI
2010
97views more  BMCBI 2010»
13 years 2 months ago
Preprocessing of gene expression data by optimally robust estimators
Background: The preprocessing of gene expression data obtained from several platforms routinely includes the aggregation of multiple raw signal intensities to one expression value...
Matthias Kohl, Hans-Peter Deigner