14 search results - page 1 / 3 » Novel computational methods for increasing PCR primer design... |
BMCBI
2008
13 years 5 months ago
2008
Background: Polymerase chain reaction (PCR) is used in directed sequencing for the discovery of novel polymorphisms. As the first step in PCR directed sequencing, effective PCR pr...
BMCBI
2006
13 years 5 months ago
2006
Background: The design of oligonucleotides and PCR primers for studying large genomes is complicated by the redundancy of sequences. The eukaryotic genomes are particularly diffic...
BMCBI
2010
13 years 5 months ago
2010
Background: Pathogen diagnostic assays based on polymerase chain reaction (PCR) technology provide high sensitivity and specificity. However, the design of these diagnostic assays...
IMSCCS
2006
IEEE
13 years 11 months ago
2006
IEEE
Background: The amplification of variable regions of immunoglobulins has become a major challenge in the cloning of antibody genes, whether from hybridoma cell lines or splenic B ...
BMCBI
2010
13 years 5 months ago
2010
Background: Affymetrix GeneChip microarrays are popular platforms for expression profiling in two types of studies: detection of differential expression computed by p-values of t-...