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BMCBI
2008
93views more  BMCBI 2008»
13 years 4 months ago
Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence
Background: In real-time PCR, it is necessary to consider the efficiency of amplification (EA) of amplicons in order to determine initial target levels properly. EAs can be deduce...
Anke Batsch, Andrea Noetel, Christian Fork, Anita ...
BMCBI
2008
132views more  BMCBI 2008»
13 years 4 months ago
Mixture models for analysis of melting temperature data
Background: In addition to their use in detecting undesired real-time PCR products, melting temperatures are useful for detecting variations in the desired target sequences. Metho...
Christoffer Nellåker, Fredrik Uhrzander, Joa...