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» Statistical analysis of real-time PCR data
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BMCBI
2005
137views more  BMCBI 2005»
13 years 3 months ago
A standard curve based method for relative real time PCR data processing
Background: Currently real time PCR is the most precise method by which to measure gene expression. The method generates a large amount of raw numerical data and processing may no...
Alexey Larionov, Andreas Krause, William Miller
BMCBI
2008
132views more  BMCBI 2008»
13 years 3 months ago
Mixture models for analysis of melting temperature data
Background: In addition to their use in detecting undesired real-time PCR products, melting temperatures are useful for detecting variations in the desired target sequences. Metho...
Christoffer Nellåker, Fredrik Uhrzander, Joa...
BMCBI
2006
240views more  BMCBI 2006»
13 years 3 months ago
Statistical analysis of real-time PCR data
Background: Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR are still lacking;...
Joshua S. Yuan, Ann Reed, Feng Chen, C. Neal Stewa...
BMCBI
2007
113views more  BMCBI 2007»
13 years 3 months ago
Statistical tools for transgene copy number estimation based on real-time PCR
Background: As compared with traditional transgene copy number detection technologies such as Southern blot analysis, real-time PCR provides a fast, inexpensive and high-throughpu...
Joshua S. Yuan, Jason N. Burris, Nathan R. Stewart...
BMCBI
2007
147views more  BMCBI 2007»
13 years 3 months ago
Statistical significance of quantitative PCR
Background: PCR has the potential to detect and precisely quantify specific DNA sequences, but it is not yet often used as a fully quantitative method. A number of data collection...
Yann Karlen, Alan McNair, Sébastien Persegu...