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    BMCBI
    2006
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    Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
    13 years 5 months ago
    Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
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    Background: In real-time PCR data analysis, the cycle threshold (CT) method is currently the gold standard. This method is based on an assumption of equal PCR efficiency in all re...
    Rasmus Goll, Trine Olsen, Guanglin Cui, Jon Florho...
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