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137views more  BMCBI 2005»
10 years 2 months ago
A standard curve based method for relative real time PCR data processing
Background: Currently real time PCR is the most precise method by which to measure gene expression. The method generates a large amount of raw numerical data and processing may no...
Alexey Larionov, Andreas Krause, William Miller
126views more  BMCBI 2008»
10 years 2 months ago
FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification
Background: FCI is an R code for analyzing data from real-time PCR experiments. This algorithm estimates standard curve features as well as nucleic acid concentrations and confide...
Paolo Verderio, Sara Pizzamiglio, Fabio Gallo, Sim...
93views more  BMCBI 2008»
10 years 2 months ago
Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence
Background: In real-time PCR, it is necessary to consider the efficiency of amplification (EA) of amplicons in order to determine initial target levels properly. EAs can be deduce...
Anke Batsch, Andrea Noetel, Christian Fork, Anita ...
10 years 7 months ago
An Object-Oriented Approach to Curves and Surfaces
class of general differentiable curves and surfaces and in turn refine this design to various parametric representations of curves and surfaces. This design includes all of the sta...
Philipp Slusallek, Reinhard Klein, Andreas Kolb, G...