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BMCBI
2010

Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles

9 years 3 months ago
Genome scale prediction of substrate specificity for acyl adenylate superfamily of enzymes based on active site residue profiles
Background: Enzymes belonging to acyl:CoA synthetase (ACS) superfamily activate wide variety of substrates and play major role in increasing the structural and functional diversity of various secondary metabolites in microbes and plants. However, due to the large sequence divergence within the superfamily, it is difficult to predict their substrate preference by annotation transfer from the closest homolog. Therefore, a large number of ACS sequences present in public databases lack any functional annotation at the level of substrate specificity. Recently, several examples have been reported where the enzymes showing high sequence similarity to luciferases or coumarate: CoA ligases have been surprisingly found to activate fatty acyl substrates in experimental studies. In this work, we have investigated the relationship between the substrate specificity of ACS and their sequence/structural features, and developed a novel computational protocol for in silico assignment of substrate prefe...
Pankaj Khurana, Rajesh S. Gokhale, Debasisa Mohant
Added 08 Dec 2010
Updated 08 Dec 2010
Type Journal
Year 2010
Where BMCBI
Authors Pankaj Khurana, Rajesh S. Gokhale, Debasisa Mohanty
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