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BMCBI
2008

Large-scale identification of polymorphic microsatellites using an in silico approach

9 years 1 months ago
Large-scale identification of polymorphic microsatellites using an in silico approach
Background: Simple Sequence Repeat (SSR) or microsatellite markers are valuable for genetic research. Experimental methods to develop SSR markers are laborious, time consuming and expensive. In silico approaches have become a practicable and relatively inexpensive alternative during the last decade, although testing putative SSR markers still is time consuming and expensive. In many species only a relatively small percentage of SSR markers turn out to be polymorphic. This is particularly true for markers derived from expressed sequence tags (ESTs). In EST databases a large redundancy of sequences is present, which may contain information on length-polymorphisms in the SSR they contain, and whether they have been derived from heterozygotes or from different genotypes. Up to now, although a number of programs have been developed to identify SSRs in EST sequences, no software can detect putatively polymorphic SSRs. Results: We have developed PolySSR, a new pipeline to identify polymorphi...
Jifeng Tang, Samantha J. Baldwin, Jeanne M. E. Jac
Added 09 Dec 2010
Updated 09 Dec 2010
Type Journal
Year 2008
Where BMCBI
Authors Jifeng Tang, Samantha J. Baldwin, Jeanne M. E. Jacobs, C. Gerard van der Linden, Roeland E. Voorrips, Jack A. M. Leunissen, Herman van Eck, Ben Vosman
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