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BMCBI
2008

The unique probe selector: a comprehensive web service for probe design and oligonucleotide arrays

13 years 3 months ago
The unique probe selector: a comprehensive web service for probe design and oligonucleotide arrays
Background: Nucleic acid hybridization, a fundamental technique in molecular biology, can be modified into very effective and sensitive methods for detecting particular targets mixed with millions of non-target sequences. Therefore, avoiding cross-hybridization is the most crucial issue for developing diagnostic methods based on hybridization. Results: To develop a probe with a high discriminating power, this study constructed a web service, the Unique Probe Selector (UPS), for customized probe design. The UPS service integrates a probe design mechanism and a scoring system for evaluating the performance of probe annealing and the uniqueness of a probe in a user-defined genetic background. Starting from an intuitive web interface, the UPS accepts a query with single or multiple sequences in fasta format. The best probe(s) for each sequence can be downloaded from result pages in a fasta or .csv format with a summary of probe characteristics. The option "Unique probe within group&q...
Shu-Hwa Chen, Chen-Zen Lo, Ming-Chi Tsai, Chao A.
Added 09 Dec 2010
Updated 09 Dec 2010
Type Journal
Year 2008
Where BMCBI
Authors Shu-Hwa Chen, Chen-Zen Lo, Ming-Chi Tsai, Chao A. Hsiung, Chung-Yen Lin
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