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BMCBI
2007

Using expression arrays for copy number detection: an example from E. coli

10 years 1 months ago
Using expression arrays for copy number detection: an example from E. coli
Background: The sequencing of many genomes and tiling arrays consisting of millions of DNA segments spanning entire genomes have made high-resolution copy number analysis possible. Microarray-based comparative genomic hybridization (array CGH) has enabled the high-resolution detection of DNA copy number aberrations. While many of the methods and algorithms developed for the analysis microarrays have focused on expression analysis, the same technology can be used to detect genetic alterations, using for example standard commercial Affymetrix arrays. Due to the nature of the resultant data, standard techniques for processing GeneChip expression experiments are inapplicable. Results: We have developed a robust and flexible methodology for high-resolution analysis of DNA copy number of whole genomes, using Affymetrix high-density expression oligonucleotide microarrays. Copy number is obtained from fluorescence signals after processing with novel normalization, spatial artifact correction,...
Dmitriy Skvortsov, Diana Abdueva, Michael E. Stitz
Added 12 Dec 2010
Updated 12 Dec 2010
Type Journal
Year 2007
Where BMCBI
Authors Dmitriy Skvortsov, Diana Abdueva, Michael E. Stitzer, Steven E. Finkel, Simon Tavaré
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