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BMCBI
2010

Using machine learning to speed up manual image annotation: application to a 3D imaging protocol for measuring single cell gene

9 years 10 months ago
Using machine learning to speed up manual image annotation: application to a 3D imaging protocol for measuring single cell gene
Background: Image analysis is an essential component in many biological experiments that study gene expression, cell cycle progression, and protein localization. A protocol for tracking the expression of individual C. elegans genes was developed that collects image samples of a developing embryo by 3-D time lapse microscopy. In this protocol, a program called StarryNite performs the automatic recognition of fluorescently labeled cells and traces their lineage. However, due to the amount of noise present in the data and due to the challenges introduced by increasing number of cells in later stages of development, this program is not error free. In the current version, the error correction (i.e., editing) is performed manually using a graphical interface tool named AceTree, which is specifically developed for this task. For a single experiment, this manual annotation task takes several hours. Results: In this paper, we reduce the time required to correct errors made by StarryNite. We ta...
Zafer Aydin, John I. Murray, Robert H. Waterston,
Added 08 Dec 2010
Updated 08 Dec 2010
Type Journal
Year 2010
Where BMCBI
Authors Zafer Aydin, John I. Murray, Robert H. Waterston, William Stafford Noble
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