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» Automated Validation of Polymerase Chain Reactions Using Amp...
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CSB
2005
IEEE
166views Bioinformatics» more  CSB 2005»
13 years 10 months ago
Automated Validation of Polymerase Chain Reactions Using Amplicon Melting Curves
PCR, the polymerase chain reaction, is a fundamental tool of molecular biology. Quantitative PCR is the goldstandard methodology for determination of DNA copy numbers, quantitatin...
Tobias P. Mann, Richard Humbert, John A. Stamatoya...
GECCO
1999
Springer
13 years 9 months ago
Reaction Temperature Constraints in DNA Computing
Using the thermodynamics of DNA melting, a technique is proposed to choose a reaction temperature for the DNA computation that minimizes the potential for mishybridizations. Adlem...
Russell J. Deaton
BMCBI
2007
94views more  BMCBI 2007»
13 years 4 months ago
Model based analysis of real-time PCR data from DNA binding dye protocols
Background: Reverse transcription followed by real-time PCR is widely used for quantification of specific mRNA, and with the use of double-stranded DNA binding dyes it is becoming...
Mariano J. Alvarez, Guillermo J. Vila-Ortiz, Maria...