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» Primer design using genetic algorithm
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BMCBI
2010
128views more  BMCBI 2010»
13 years 5 months ago
MPprimer: a program for reliable multiplex PCR primer design
Background: Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a f...
Zhiyong Shen, Wubin Qu, Wen Wang, Yiming Lu, Yongh...
BMCBI
2005
84views more  BMCBI 2005»
13 years 5 months ago
Information theory-based algorithm for in silico prediction of PCR products with whole genomic sequences as templates
Background: A new algorithm for assessing similarity between primer and template has been developed based on the hypothesis that annealing of primer to template is an information ...
Youfang Cao, Lianjie Wang, Kexue Xu, Chunhai Kou, ...
EMO
2006
Springer
117views Optimization» more  EMO 2006»
13 years 7 months ago
Multiplex PCR Assay Design by Hybrid Multiobjective Evolutionary Algorithm
Abstract. Multiplex Polymerase Chain Reaction (PCR) assay is to amplify multiple target DNAs simultaneously using different primer pairs for each target DNA. Recently, it is widely...
In-Hee Lee, Soo-Yong Shin, Byoung-Tak Zhang
BMCBI
2008
136views more  BMCBI 2008»
13 years 5 months ago
LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens
Background: Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen detection chips currently utili...
Wah-Heng Lee, Christopher W. Wong, Wan Yee Leong, ...
BMCBI
2006
144views more  BMCBI 2006»
13 years 5 months ago
methBLAST and methPrimerDB: web-tools for PCR based methylation analysis
Background: DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methy...
Filip Pattyn, Jasmien Hoebeeck, Piet Robbrecht, Ev...