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BMCBI
2006

methBLAST and methPrimerDB: web-tools for PCR based methylation analysis

8 years 10 months ago
methBLAST and methPrimerDB: web-tools for PCR based methylation analysis
Background: DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methylation assays on bisulphite modified DNA heralded a breakthrough in speed and sensitivity for gene methylation analysis. Despite this technological advancement, these approaches require a cumbersome gene by gene primer design and experimental validation. Bisulphite DNA modification results in sequence alterations (all unmethylated cytosines are converted into uracils) and a general sequence complexity reduction as cytosines become underrepresented. Consequently, standard BLAST sequence homology searches cannot be applied to search for specific methylation primers. Results: To address this problem we developed methBLAST, a sequence similarity search program, based on the original BLAST algorithm but querying in silico bisulphite modified genome sequences to evaluate oligonucleotide sequence similarities. Apart...
Filip Pattyn, Jasmien Hoebeeck, Piet Robbrecht, Ev
Added 10 Dec 2010
Updated 10 Dec 2010
Type Journal
Year 2006
Where BMCBI
Authors Filip Pattyn, Jasmien Hoebeeck, Piet Robbrecht, Evi Michels, Anne De Paepe, Guy Bottu, David Coornaert, Robert Herzog, Frank Speleman, Jo Vandesompele
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