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2003
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Group Testing With DNA Chips: Generating Designs and Decoding Experiments

9 years 4 months ago
Group Testing With DNA Chips: Generating Designs and Decoding Experiments
DNA microarrays are a valuable tool for massively parallel DNA-DNA hybridization experiments. Currently, most applications rely on the existence of sequence-specific oligonucleotide probes. In large families of closely related target sequences, such as different virus subtypes, the high degree of similarity often makes it impossible to find a unique probe for every target. Fortunately, this is unnecessary. We propose a microarray design methodology based on a group testing approach. While probes might bind to multiple targets simultaneously, a properly chosen probe set can still unambiguously distinguish the presence of one target set from the presence of a different target set. Our method is the first one that explicitly takes cross-hybridization and experimental errors into account while accommodating several targets. The approach consists of three steps: (1) Pre-selection of probe candidates, (2) Generation of a suitable group testing design, and (3) Decoding of hybridization re...
Alexander Schliep, David C. Torney, Sven Rahmann
Added 04 Jul 2010
Updated 04 Jul 2010
Type Conference
Year 2003
Where CSB
Authors Alexander Schliep, David C. Torney, Sven Rahmann
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