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ISMB
1997

Increasing Consensus Accuracy in DNA Fragment Assemblies by Incorporating Fluorescent Trace Representations

13 years 5 months ago
Increasing Consensus Accuracy in DNA Fragment Assemblies by Incorporating Fluorescent Trace Representations
We present a new method for determining the consensus sequence in DNA fragment assemblies. The new method, Trace-Evidence, directly incorporates aligned ABI trace information into consensus calculations via our previously described representation, Trace-Data Classifications. The new method extracts and sums evidence indicated by the representation to determine consensus calls. Using the Trace-Evidence method results in automatically produced consensus sequences that are more accurate and less ambiguous than those produced with standard majorityvoting methods. Additionally, these improvements are achieved with less coverage than required by the standard methods – using Trace-Evidence and a coverage of only three, errorrates are as low as those with a coverage of over ten sequences.
Carolyn F. Allex, Schuyler F. Baldwin, Jude W. Sha
Added 01 Nov 2010
Updated 01 Nov 2010
Type Conference
Year 1997
Where ISMB
Authors Carolyn F. Allex, Schuyler F. Baldwin, Jude W. Shavlik, Frederick R. Blattner
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