Standardized high-throughput evaluation of cell-based compound screens

Background: High-throughput screening of pharmaceutical compound activity in tissue culture experiments requires time-consuming repeated analysis of the large amounts of data generated. Automation of the evaluation procedure and assessment of measurement accuracy can save time and improve the comparability of results. Results: We present a tool for simultaneous evaluation of an arbitrary number of compound screens including a standardized statistical validation. It is provided as a novel R package with a Tcl/ Tk-based GUI for convenient use in the lab and runs on usual platforms like Linux, Windows and Mac OS. In a compound screen of lung cancer cells, the tool was successfully and efficiently applied for data analysis. Conclusion: The package provides an efficient and intuitive platform for automatic evaluation of compound screens, improving the performance and standardization of data analysis. Background Cell-based screening of the cytotoxic activity of chemical compounds in cancer ...

• Type: Journal
• Year: 2008
• Where: BMCBI
• Authors: Peter Frommolt, Roman K. Thomas
• Keywords: BMCBI 2008, Cancer Cell, Compound Screens, Evaluation