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BMCBI
2005

A stepwise framework for the normalization of array CGH data

11 years 5 months ago
A stepwise framework for the normalization of array CGH data
Background: In two-channel competitive genomic hybridization microarray experiments, the ratio of the two fluorescent signal intensities at each spot on the microarray is commonly used to infer the relative amounts of the test and reference sample DNA levels. This ratio may be influenced by systematic measurement effects from non-biological sources that can introduce biases in the estimated ratios. These biases should be removed before drawing conclusions about the relative levels of DNA. The performance of existing gene expression microarray normalization strategies has not been evaluated for removing systematic biases encountered in array-based comparative genomic hybridization (CGH), which aims to detect single copy gains and losses typically in samples with heterogeneous cell populations resulting in only slight shifts in signal ratios. The purpose of this work is to establish a framework for correcting the systematic sources of variation in high density CGH array images, while ma...
Mehrnoush Khojasteh, Wan L. Lam, Rabab Kreidieh Wa
Added 15 Dec 2010
Updated 15 Dec 2010
Type Journal
Year 2005
Where BMCBI
Authors Mehrnoush Khojasteh, Wan L. Lam, Rabab Kreidieh Ward, Calum MacAulay
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