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BMCBI
2005

SUPERFICIAL - Surface mapping of proteins via structure-based peptide library design

10 years 1 months ago
SUPERFICIAL - Surface mapping of proteins via structure-based peptide library design
Background: The determination of protein surfaces and the detection of binding sites are essential to our understanding of protein-protein interactions. Such binding sites can be characterised as linear and non-linear, the non-linear sites being prevailant. Conventional mapping techniques with arrays of synthetic peptides have limitations with regard to the location of discontinuous or non-linear binding sites of proteins. Results: We present a structure-based approach to the design of peptide libraries that mimic the whole surface or a particular region of a protein. Neighbouring sequence segments are linked by short spacers to conserve local conformation. To this end, we have developed SUPERFICIAL, a program that uses protein structures as input and generates library proposals consisting of linear and non-linear peptides. This process can be influenced by a graphical user interface at different stages, from the surface computation up to the definition of spatial regions. Conclusion:...
Andrean Goede, Ines S. Jaeger, Robert Preissner
Added 15 Dec 2010
Updated 15 Dec 2010
Type Journal
Year 2005
Where BMCBI
Authors Andrean Goede, Ines S. Jaeger, Robert Preissner
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