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CSB
2005
IEEE

Deformable Modeling for Improved Calculation of Molecular Velocities from Single-Particle Tracking

13 years 9 months ago
Deformable Modeling for Improved Calculation of Molecular Velocities from Single-Particle Tracking
Single-particle tracking provides a powerful technique for measuring dynamic cellular processes on the level of individual molecules. Much recent work has been devoted to using single particle tracking to measure long-range movement of particles on the cell surface, including methods for automated localization and tracking of particles [1-3]. However, most particle tracking studies to date ignore cell surface curvature and dynamic cellular deformation, factors frequently present in physiologically relevant situations. In this report, we perform quantitative evaluation of single-particle tracking on curved and deforming cell surfaces. We also introduce a new hybrid method that uses non-rigid cellular modeling for improved computation of single-particle tracking trajectories on the surfaces of cells undergoing deformation. This method combines single-molecule and bulk fluorescence measurements in an automated manner to enable more accurate and robust characterization of dynamic cell phy...
Peter M. Kasson, Mark M. Davis, Axel T. Brüng
Added 24 Jun 2010
Updated 24 Jun 2010
Type Conference
Year 2005
Where CSB
Authors Peter M. Kasson, Mark M. Davis, Axel T. Brünger
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