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2005
IEEE

Maximum Sequence Alignment Fails to Predict Off-targeted Gene Regulation by RNAi

12 years 7 months ago
Maximum Sequence Alignment Fails to Predict Off-targeted Gene Regulation by RNAi
We have employed various sequence alignment algorithms and scoring techniques to determine whether current computational tools accurately predict genes that will be off-targeted by the RNA interference (RNAi) pathway. Our studies show that distributions of maximum alignment scores for off-targeted and untargeted genes are statistically indistinguishable, indicating that maximum complementarity by itself is an unsatisfactory predictor of off-targeting. Interestingly, a highly significant association was observed between off-targeting and exact complementarity between the seed region (bases 2-7) of siRNA and their off-targeted genes. This pattern has been previously recognized in microRNA-mediated gene knockdown and suggests a distinctive role for the 5’ terminus of these strands in RNAi-triggered gene suppression.
Amanda Birmingham, Emily M. Anderson, William S. M
Added 24 Jun 2010
Updated 24 Jun 2010
Type Conference
Year 2005
Where CSB
Authors Amanda Birmingham, Emily M. Anderson, William S. Marshall, Anastasia Khvorova
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