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BMCBI
2005

Genome-wide identification of the regulatory targets of a transcription factor using biochemical characterization and computatio

13 years 2 months ago
Genome-wide identification of the regulatory targets of a transcription factor using biochemical characterization and computatio
Background: A major challenge in computational genomics is the development of methodologies that allow accurate genome-wide prediction of the regulatory targets of a transcription factor. We present a method for target identification that combines experimental characterization of binding requirements with computational genomic analysis. Results: Our method identified potential target genes of the transcription factor Ndt80, a key transcriptional regulator involved in yeast sporulation, using the combined information of binding affinity, positional distribution, and conservation of the binding sites across multiple species. We have also developed a mathematical approach to compute the false positive rate and the total number of targets in the genome based on the multiple selection criteria. Conclusion: We have shown that combining biochemical characterization and computational genomic analysis leads to accurate identification of the genome-wide targets of a transcription factor. The me...
Emmitt R. Jolly, Chen-Shan Chin, Ira Herskowitz, H
Added 15 Dec 2010
Updated 15 Dec 2010
Type Journal
Year 2005
Where BMCBI
Authors Emmitt R. Jolly, Chen-Shan Chin, Ira Herskowitz, Hao Li
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