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» A standard curve based method for relative real time PCR dat...
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78
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BMCBI
2005
137views more  BMCBI 2005»
14 years 10 months ago
A standard curve based method for relative real time PCR data processing
Background: Currently real time PCR is the most precise method by which to measure gene expression. The method generates a large amount of raw numerical data and processing may no...
Alexey Larionov, Andreas Krause, William Miller
111
Voted
BMCBI
2010
143views more  BMCBI 2010»
14 years 10 months ago
Shape based kinetic outlier detection in real-time PCR
Background: Real-time PCR has recently become the technique of choice for absolute and relative nucleic acid quantification. The gold standard quantification method in real-time P...
Davide Sisti, Michele Guescini, Marco B. L. Rocchi...
93
Voted
BMCBI
2006
240views more  BMCBI 2006»
14 years 10 months ago
Statistical analysis of real-time PCR data
Background: Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR are still lacking;...
Joshua S. Yuan, Ann Reed, Feng Chen, C. Neal Stewa...
106
Voted
BMCBI
2007
94views more  BMCBI 2007»
14 years 10 months ago
Model based analysis of real-time PCR data from DNA binding dye protocols
Background: Reverse transcription followed by real-time PCR is widely used for quantification of specific mRNA, and with the use of double-stranded DNA binding dyes it is becoming...
Mariano J. Alvarez, Guillermo J. Vila-Ortiz, Maria...
87
Voted
BMCBI
2008
126views more  BMCBI 2008»
14 years 10 months ago
FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification
Background: FCI is an R code for analyzing data from real-time PCR experiments. This algorithm estimates standard curve features as well as nucleic acid concentrations and confide...
Paolo Verderio, Sara Pizzamiglio, Fabio Gallo, Sim...