Primer approximation multiplex PCR (PAMP) is a new experimental protocol for efficiently assaying structural variation in genomes. PAMP is particularly suited to cancer genomes w...
Ali Bashir, Yu-Tsueng Liu, Benjamin J. Raphael, De...
Abstract. Multiplex Polymerase Chain Reaction (PCR) assay is to amplify multiple target DNAs simultaneously using different primer pairs for each target DNA. Recently, it is widely...
Background: DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methy...
Filip Pattyn, Jasmien Hoebeeck, Piet Robbrecht, Ev...
Background: Direct synthesis of genes is rapidly becoming the most efficient way to make functional genetic constructs and enables applications such as codon optimization, RNAi re...
Alan Villalobos, Jon E. Ness, Claes Gustafsson, Je...
Background: The amplification of variable regions of immunoglobulins has become a major challenge in the cloning of antibody genes, whether from hybridoma cell lines or splenic B ...