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BIOINFORMATICS
2007
76views more  BIOINFORMATICS 2007»
13 years 6 months ago
Optimization of primer design for the detection of variable genomic lesions in cancer
Primer approximation multiplex PCR (PAMP) is a new experimental protocol for efficiently assaying structural variation in genomes. PAMP is particularly suited to cancer genomes w...
Ali Bashir, Yu-Tsueng Liu, Benjamin J. Raphael, De...
EMO
2006
Springer
117views Optimization» more  EMO 2006»
13 years 8 months ago
Multiplex PCR Assay Design by Hybrid Multiobjective Evolutionary Algorithm
Abstract. Multiplex Polymerase Chain Reaction (PCR) assay is to amplify multiple target DNAs simultaneously using different primer pairs for each target DNA. Recently, it is widely...
In-Hee Lee, Soo-Yong Shin, Byoung-Tak Zhang
BMCBI
2006
144views more  BMCBI 2006»
13 years 6 months ago
methBLAST and methPrimerDB: web-tools for PCR based methylation analysis
Background: DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methy...
Filip Pattyn, Jasmien Hoebeeck, Piet Robbrecht, Ev...
BMCBI
2006
117views more  BMCBI 2006»
13 years 6 months ago
Gene Designer: a synthetic biology tool for constructing artificial DNA segments
Background: Direct synthesis of genes is rapidly becoming the most efficient way to make functional genetic constructs and enables applications such as codon optimization, RNAi re...
Alan Villalobos, Jon E. Ness, Claes Gustafsson, Je...
IMSCCS
2006
IEEE
14 years 14 days ago
Degenerated Primer Design to Amplify the Heavy Chain Variable Region from Immunoglobulin cDNA
Background: The amplification of variable regions of immunoglobulins has become a major challenge in the cloning of antibody genes, whether from hybridoma cell lines or splenic B ...
Wang Ying, Chen Wei, Li Xu, Cheng Bing