5 search results - page 1 / 1 » Primique: automatic design of specific PCR primers for each ... |
BMCBI
2007
13 years 6 months ago
2007
Background: In many contexts, researchers need specific primers for all sequences in a family such that each primer set amplifies only its target sequence and none of the others, ...
BMCBI
2006
13 years 6 months ago
2006
Background: The design of oligonucleotides and PCR primers for studying large genomes is complicated by the redundancy of sequences. The eukaryotic genomes are particularly diffic...
CSB
2003
IEEE
13 years 11 months ago
2003
IEEE
This paper describes a new strategy for designing degenerate primers for a given multiple alignment of amino acid sequences. Degenerate primers are useful for amplifying homologou...
BMCBI
2006
13 years 6 months ago
2006
Background: DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methy...
BMCBI
2010
13 years 6 months ago
2010
Background: Repeat-rich regions such as centromeres receive less attention than their gene-rich euchromatic counterparts because the former are difficult to assemble and analyze. ...