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115
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BMCBI
2011
14 years 7 months ago
The impact of quantitative optimization of hybridization conditions on gene expression analysis
Background: With the growing availability of entire genome sequences, an increasing number of scientists can exploit oligonucleotide microarrays for genome-scale expression studie...
Peter Sykacek, David P. Kreil, Lisa A. Meadows, Ri...
93
Voted
BMCBI
2010
193views more  BMCBI 2010»
15 years 16 days ago
Probe set filtering increases correlation between Affymetrix GeneChip and qRT-PCR expression measurements
Background: Affymetrix GeneChip microarrays are popular platforms for expression profiling in two types of studies: detection of differential expression computed by p-values of t-...
Jakub Mieczkowski, Magdalena E. Tyburczy, Michal D...
113
Voted
BMCBI
2006
99views more  BMCBI 2006»
15 years 14 days ago
A proposed metric for assessing the measurement quality of individual microarrays
Background: High-density microarray technology is increasingly applied to study gene expression levels on a large scale. Microarray experiments rely on several critical steps that...
Kyoungmi Kim, Grier P. Page, T. Mark Beasley, Step...
109
Voted
BMCBI
2008
138views more  BMCBI 2008»
15 years 15 days ago
Combining transcriptional datasets using the generalized singular value decomposition
Background: Both microarrays and quantitative real-time PCR are convenient tools for studying the transcriptional levels of genes. The former is preferable for large scale studies...
Andreas W. Schreiber, Neil J. Shirley, Rachel A. B...
112
Voted
BMCBI
2006
125views more  BMCBI 2006»
15 years 13 days ago
Evaluating different methods of microarray data normalization
Background: With the development of DNA hybridization microarray technologies, nowadays it is possible to simultaneously assess the expression levels of thousands to tens of thous...
André Fujita, João Ricardo Sato, Leo...