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BMCBI
2010

Empirical Bayes Analysis of Sequencing-based Transcriptional Profiling without Replicates

13 years 3 months ago
Empirical Bayes Analysis of Sequencing-based Transcriptional Profiling without Replicates
Background: Recent technological advancements have made high throughput sequencing an increasingly popular approach for transcriptome analysis. Advantages of sequencing-based transcriptional profiling over microarrays have been reported, including lower technical variability. However, advances in technology do not remove biological variation between replicates and this variation is often neglected in many analyses. Results: We propose an empirical Bayes method, titled Analysis of Sequence Counts (ASC), to detect differential expression based on sequencing technology. ASC borrows information across sequences to establish prior distribution of sample variation, so that biological variation can be accounted for even when replicates are not available. Compared to current approaches that simply tests for equality of proportions in two samples, ASC is less biased towards highly expressed sequences and can identify more genes with a greater log fold change at lower overall abundance. Conclus...
Zhijin Wu, Bethany D. Jenkins, Tatiana A. Rynearso
Added 24 Dec 2010
Updated 24 Dec 2010
Type Journal
Year 2010
Where BMCBI
Authors Zhijin Wu, Bethany D. Jenkins, Tatiana A. Rynearson, Sonya T. Dyhrman, Mak A. Saito, Melissa Mercier, LeAnn P. Whitney
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